Main Article Content
The main objective of this study was to clarify the best concentration of the indole-3-butyric acid (IBA) in order to induce the formation of strong roots of Arbutus pavarii Pamp, an endangered plant in the El-Jabel El-Akhdar region in Libya. A study was carried out to find a protocol for its in vitro propagation. The present paper aimed to investigate the effects of different concentrations of IBA plant growth regulator on the rooting. Three weeks old seedlings obtained with in vitro germination were transferred to Murashige and Skoog (M&S) roots induction medium supplemented with different concentrations of IBA (0, 1, 1.5 and 2 mg L-1). The highest response was obtained with the M&S medium half-strength supplemented with IBA 1 mg L-1 concentration. All the measured growth indicators (rooting percentage, root length and dry weight) significantly enhanced when using this concentration.
Elshatshat S. Biological conservation of the endemic Arbutus pavarii Pamp: Seed germination as attempt, International Journal of Human Geography and Environmental Studies. 2009;1(1):20-22.
El-Darier SM, El-Mogaspi FM. Ethnobotany and relative importance of some endemic plant species at El-Jabal El-Akhdar Region (Libya). World Journal of Agricultural Sciences. 2009;5(3):353-360.
Kabiel HF, Hegazy AK, Lovett-Doust L, Al-Rowaily SL, El Borki A. Demography of the threatened endemic shrub, Arbutus pavarii, in the Al-Akhdar mountainous landscape of Libya. J. For. Res. 2016;27(3).
Keith HG. Preliminary check list of Libyan flora. Ministry of agriculture, Tripoli, Libya; 1965.
Pisi A. Strawberry virus diseases. EPPO Bulletin. 2008;16(2):353-358.
Thorpe TA. The current status of plant tissue culture. In: Bhojwani SS (ed) Developments in crop science 19. Plant tissue culture: Applications and limitations, Elsevier, Amsterdam. 190;1–33.
Plana D, Álvarez M, Florido M, Lara Ry, Núñez M. Efecto del Biobras 6 en la morfogénesis in vitro del tomate (Lycopersicon esculentum, Mill) var. Amalia. Cultivos Tropicales. 2002;23(2): 21-25.
Rout GR. Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) Kuntze and associated biochemical changes. Plant Growth Regulation. 2006;48(2).
Pooja Goyal, Sumita Kachhwaha SL. Kothari. Micropropagation of Pithecellobium dulce (Roxb.) Benth — A multipurpose leguminous tree and assessment of genetic fidelity of micropropagated plants using molecular markers. Physiol Mol Biol Plants. 2012; 18(2).
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 1962;15:473–497
Rabha M. Mansur. Effect of chitosan on in vitro seed germination and seedling development of soybean (Glycine max L.) Global Journal of Plant Science 2. 2017; 16-21
Boxus P. Micropropagation of strawberry via axillary shoot proliferation, In: Plant Cell Culture Protocols, Methods in Molecular Biology, Part III, Plant Propagation in vitro, Hall RD (ed,) Humana Press Inc, Totowa NJ. 1999;111:103-114.
Debnath SC. Strawberry sepal: Another explant for thidiazuron-induced adventitious shoot regeneration. In Vitro Cell. Graham J, McNicol RJK. 2005;41: 671-676.
Graham J, McNicol RJ, Greig K. Transgenic apples and strawberries: Advances in transformation, introduction of genes for insect resistance and field studies of tissue cultured plants. Ann Appl Biol. 1995;127:163-173.
Rugini E, Orlando R. High efficiency shoot regeneration from calluses of strawberry (Fragaria × ananassa Duch.) stipules of in vitro cultures. Journal of Horticultural Science. 1992;67:577-582
Passey AJ, Barrett KJ, James DJ. Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria x ananassa Duch.) using a range of explant types. Plant Cell Rep. 2003;21: 397-401.
Ashrafuzz A, Faisal SM, Yadav D, Khanam D, Raihan F. Miciropagation of strawberry (Fragaria ananassa) through runner culture Bangladesh J. Agril. Res. 2013;38 (3):467-472.
Emarah. Factors affecting propagation of strawberry (Fragaria spp,) through tissue cultures, J, Product, & Dev. 2008;13(1): 191-212.
Guatam H, Kaur R, Shama DR, Neetu T. A comparative study on in vitro and ex vitro rooting of micropropagated shoots of strawberry (Fragaria × ananassa Duch,), Plant Cell Biotechnology and Molecular Biology. 2001;2(3/4):149-152.
Mereti M, Grigoriadou K, Leventakis N. In vitro rooting of strawberry tree, (Arbutus unedo L,) in medium solidified by peat, perlite mixture in combination with agar, Acta Hort. 2003;616: 607-609.
Haddadi F, Abd Aziz M, Abd Rashid A, Kamaladid H. Micro propagation of Strawberry cv. Camarosa: Prolific shoot regeneration from in vitro shoot tips using thidiazuron with N6- benzylamino- purine. Hort Scince. 2010;45(3): 453-456.